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The present study analyzed changes in Wnt3a expression during differentiation of adipose-derived stem cells into cholinergic neurons.Immunocytochemistry and immunofluorescence revealed significantly increased nestin,neuron-specific enolase,microtubule-associated protein 2,and choline acetyltransferase expression in adipose-derived stem cells isolated from Sprague-Dawley rats and cultured in vitro in neural-induced medium.These expressions increased with prolonged induction time.Real-time reverse transcription-PCR and western blot assay results demonstrated significantly increased choline acetyltransferase and Wnt3a protein and mRNA expressions,respectively,in adipose-derived stem cells following induction.Choline acetyltransferase expression positively correlated with Wnt3a protein and mRNA expressions.These results demonstrated that neural-induced medium induced differentiation of adipose-derived stem cells into cholinergic neuronal-like cells,with subsequent increased Wnt3a expression.
The present study analyzing changes in in Wnt3a expression during differentiation of adipose-derived stem cells into cholinergic neurons. Immunocytochemistry and immunofluorescence of lumen revealed an increased nestin, neuron-specific enolase, microtubule-associated protein 2, and choline acetyltransferase expression in adipose-derived stem cells isolated from Sprague-Dawley rats and cultured in vitro in neural-induced medium. These expressions increased with prolonged induction of time. Real-time reverse transcription-PCR and western blot assayeda- tion showed significantly increased choline acetyltransferase and Wnt3a protein and mRNA expressions, respectively, in adipose-derived stem cells following induction. Cholinergic acetyltransferase expression associated with Wnt3a protein and mRNA expressions. These results of that neural-induced medium induced differentiation of adipose-derived stem cells into cholinergic neuronal-like cells, with subsequent increased Wnt3a expressio n.