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目的:测定转黄芪毛状根中黄芪甲苷含量。方法:利用HPLC-ELSD法测黄芪甲苷含量。结果:转透明颤菌血红蛋白基因(Vitreoscilla Haemoglobin gene,vgb)黄芪毛状根、非转基因黄芪毛状根和山西产黄芪药材黄芪甲苷含量差异大。结论:转vgb黄芪毛状根黄芪甲苷含量远远高于非转基因黄芪毛状根和黄芪药材。
Objective: To determine the content of Astragaloside Ⅳ in the hairy root of Astragalus. Methods: The content of astragaloside Ⅳ was determined by HPLC-ELSD. Results: The contents of Astragaloside Ⅳ in astragalus root, non-transgenic Astragalus hairy root and Astragalus membranaceus in Shanxi were significantly different after transfered Vitreoscilla hemoglobin gene (vgb). Conclusion: The content of astragaloside IV in the hairy roots of Astragalus mongholicus transplanted with vgb is much higher than that of the non-transgenic Astragalus mongholicus and Astragalus mongholicus.