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目的建立雪胆胃肠丸中延胡索乙素含量测定的方法。方法采用Hypersil ODS2柱(250 mm×4.6 mm,5μm),乙腈-0.1%磷酸溶液(三乙胺调pH至6.0)(40∶60)为流动相;流速1.0 mL.min-1;检测波长225 nm;柱温:25℃。结果延胡索乙素在4.8~48.0μg.mL-1内与其峰面积有良好的线性关系,其平均回收率为99.82%,RSD为1.00%(n=6)。结论所建方法简便易行、准确可靠,可用于雪胆胃肠丸中延胡索乙素的含量测定。
Objective To establish a method for the determination of tetrahydropalmatine in Xuebianweichang pill. Methods The mobile phase consisted of Hypersil ODS2 column (250 mm × 4.6 mm, 5 μm), acetonitrile-0.1% phosphoric acid solution (triethylamine adjusted to pH 6.0) (40:60), flow rate 1.0 mL · min- nm; column temperature: 25 ℃. Results Tetrahydropalmatine had a good linear relationship with its peak area in the range of 4.8 ~ 48.0μg.mL-1, with an average recovery of 99.82% and a RSD of 1.00% (n = 6). Conclusion The method is simple, accurate and reliable and can be used for the determination of tetrahydropalmatine in Xuebian Gastrointestinal Pills.