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通过草莓花药培养诱导单倍体再生植株,是单倍体育种的关键,本研究以栽培广泛、适应性强的丹东鸡冠为试材,用单核期花药进行接种,经愈伤组织、不定芽、无根苗、生根等各阶段,已诱导出单倍体植株。我们在同类研究的基础上筛选出了表面灭菌的最佳方法,在诱导愈伤组织不定芽和生根过程中,筛选出了生长素、细胞分裂素适宜比值和最佳培养基配方,从而为草莓单倍体育种奠定了良好的基础。
Induction of haploid plant regeneration by strawberry anther culture is the key to haploid breeding. In this study, a wide range of adaptable Dandong cockscomb were used as experimental materials, which were inoculated with mononuclear anthers. The callus, adventitious buds , No root seedlings, rooting and other stages, has induced haploid plants. On the basis of similar studies, we screened out the best method of surface sterilization. During the induction of adventitious buds and rooting of callus, we screened the appropriate ratio of auxin, cytokinin and the best medium formula Strawberry haploid breeding has laid a good foundation.