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目的:可德兰多糖是目前发现的唯一不含分支的线性β-1,3-葡聚糖,由于难溶于水而限制其生物活性的研究,为此对可德兰寡糖的制备及其组分进行研究。方法:采用化学法酸解可德兰多糖。结果:通过红外光谱与基质辅助激光解析/电离飞行时间质谱(MALDI-TOF MS)的检测,结果表明:可德兰多糖降解后结构未发生变化,降解产物的聚合度为2～19,其中聚合度为5的成分含量最高;高效阴离子交换色谱脉冲安培检测法分析结果表明:反应温度105℃、反应时间150～180min是降解可德兰寡糖的合理反应条件,降解效果最佳。结论:本研究建立的方法可简单、高效的降解可德兰多糖,制备其寡糖。
OBJECTIVE: Codulan polysaccharide is the only unbranched linear β-1,3-glucan that has been found to be limited in biological activity because of its poor solubility in water. To this end, Its components are studied. Methods: Chemically acidolyzed guandu polysaccharides. Results: The results of FTIR and MALDI-TOF MS showed that the structure of the degradable polysaccharides did not change after degradation, the degree of polymerization of degradation products was from 2 to 19, The results showed that the reaction temperature was 105 ℃ and the reaction time was 150 ~ 180min, which was the reasonable reaction condition for the degradation of the radiant oligosaccharides, and the degradation was the best. Conclusion: The method established in this study can easily and efficiently degrade kadsuran polysaccharide and prepare its oligosaccharide.