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By the use of the fluorescent retrograde tracer, Fast Blue (FB), in combination with monoamine fluorescence histochemistry, the origin of monoaminergic input to the region of the nucleus raphe magnus (NRM) was investigated in the rat. After mieroinjection of FB into the NRM, a great number of FB-laboled NA-containing cells were found in the region of the nucleus reticularis lateralis (corresponding to A1 NA areas), the reticular formation just dorsolateral to the nucleus olivaris inferior (corresponding to the A3 NA area), the ventral part of the locus coeruleus (A6 NA area), and the lateral parts of the nucleus raphe dorsalis (B7 area). In the other NA cell groups of the brain stem, FB-labeled cells could not be observed. Serotoninergic input originating from nucleus raphe obscurus (B2), nucleus raphe pallidus (B1) and nucleus raphe pontis (BS-B6) to the NRM was also observed. A large number of cells containing serotonin (5-HT) in the B2 and B6 areas were labeled by FB, while only a few FB-labeled 5-HT
By the use of the fluorescent retrograde tracer, Fast Blue (FB), in combination with monoamine fluorescence histochemistry, the origin of monoaminergic input to the region of the nucleus raphe magnus (NRM) was investigated in the rat. After mieroinjection of FB into the NRM, a great number of FB-laboratories NA-containing cells were found in the region of the nucleus reticularis lateralis (corresponding to A1 NA areas), the reticular formation just dorsolateral to the nucleus olivaris inferior (corresponding to the A3 NA area) the ventral part of the locus coeruleus (A6 NA area), and the lateral parts of the nucleus raphe dorsalis (B7 area). In the other NA cell groups of the brain stem, FB-labeled cells could not be observed. From nucleus raphe obscurus (B2), nucleus raphe pallidus (B1) and nucleus raphe pontis (BS-B6) to the NRM was also observed. A large number of cells containing serotonin (5-HT) by FB, while only af ew FB-labeled 5-HT