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应用高效液相色谱-串联质谱法测定鸡肉产品中金刚烷胺的残留量。样品经乙腈提取,以乙腈-水(3+7)溶液作为定容溶液。Phenomenex Kinetex C18色谱柱为分离柱,以不同体积比的0.1%(体积分数)甲酸溶液和乙腈为流动相进行梯度洗脱,采用电喷雾正离子源选择反应监测模式检测。采用基质匹配曲线校正。金刚烷胺的质量浓度在0.005~0.1mg·L-1范围内与其峰面积呈线性关系,检出限(3S/N)为1.0μg·kg-1。以空白样品为基体进行加标回收试验,所得回收率在82.5%~94.0%之间,测定值的相对标准偏差(n=6)在6.3%~11%之间。
Determination of Adamantane Residues in Chicken Products by High Performance Liquid Chromatography - Tandem Mass Spectrometry. Samples were extracted with acetonitrile, acetonitrile - water (3 +7) solution as a constant volume solution. The Phenomenex Kinetex C18 column was used as a separation column. The mobile phase was eluted with 0.1% (volume fraction) formic acid solution in different volumes and acetonitrile as the mobile phase. The electrospray positive ion source was used to select the reaction monitoring mode. Matrix matching curve correction. The concentration of amantadine in the range of 0.005 ~ 0.1 mg · L-1 showed a linear relationship with the peak area. The detection limit (3S / N) was 1.0 μg · kg-1. The spiked samples were taken as the matrix and the recoveries were between 82.5% and 94.0%. The relative standard deviations (n = 6) of the measured values ranged from 6.3% to 11%.