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用单克隆抗体双位点夹心一步法测定了胃癌、食管癌、胃溃疡和各种胃炎患者胃液癌胚抗原含量。结果显示,胃癌组胃液癌胚抗原显著高于食管癌(P<0.05)、溃疡组(P<0.001)和胃炎组(P<0.001);胃液癌胚抗原测定诊断胃癌的灵敏度、特异性和总符合率即准确度分别为82.6％、88.5％和87.1％。提示胃液癌胚抗原检测诊断胃癌的价值远优于血清癌胚抗原的测定;本缓冲稀释法简便、快速,检测灵敏度达0.4ng/ml。1.5h可批量报告结果。
One-step sandwich method with monoclonal antibody was used to determine the carcinoembryonic antigen content in gastric juice, esophageal cancer, gastric ulcer, and various gastritis patients. The results showed that the gastric carcinoembryonic antigen in gastric cancer group was significantly higher than that in esophageal cancer (P<0.05), ulcer group (P<0.001) and gastritis group (P<0.001); the sensitivity, specificity, and totality of gastric carcinoembryonic antigen assay in the diagnosis of gastric cancer. The accuracy rate of accuracy is 82.6%, 88.5% and 87.1% respectively. The value of gastric carcinoembryonic antigen detection in the diagnosis of gastric cancer is much better than that of serum carcinoembryonic antigen. The buffer dilution method is simple and rapid, and the detection sensitivity is 0.4ng/ml. 1.5h can batch report results.