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Aim:Polypeptide from Chlamys farreri (PCF,molecular mass is 879) is a newmarine polypeptide compound isolated from Chlamys farreri.This study investi-gates the possible protective roles and the mechanism of PCF against ultravioletB (UVB)-induced apoptosis in murine thymocytes.Methods:The rate of apoptosisand caspase-3 activation was measured by flow cytometry.The expression ofstress-response genes c-fos and c-jun was observed by RT-PCR.Western blotanalysis was performed to determine the release of cytochrome c.Results:It wasfound that UVB induced murine thymocyte death.The cells treated with UVBshowed an increase in cytochrome c release,caspase-3 activity,as well as in theexpression of c-fos and c-jun.In addition,all were involved in UVB-induced cellapoptosis.Conclusion:Our present observations pointed to the ability of PCF toavert UVB-induced apoptosis in thymocytes by modulating c-fos and c-junexpression,cytochrome c release,and the consequent activation of caspase-3,which were essential components of the UV-induced cell apoptotic pathway.Theresults suggested that PCF is a promising protective substance against UVradiation.
Aim: Polypeptide from Chlamys farreri (PCF, molecular mass is 879) is a newmarine polypeptide compound isolated from Chlamys farreri. This study in vesti-gates the possible protective roles and the mechanism of PCF against ultraviolet B (UVB) -induced apoptosis in murine thymocytes. Methods: The rate of apoptosis and caspase-3 activation was measured by flow cytometry. The expression of stress-response genes c-fos and c-jun was observed by RT-PCR. Western blot analysis was performed to determine the release of cytochrome c. Results: It wasfound that UVB induced murine thymocyte death. The cells treated with UVB showed an increase in cytochrome c release, caspase-3 activity, as well as in the expression of c-fos and c-jun. Addition, all were involved in UVB-induced cellapoptosis.Conclusion: Our presentations pointed to the ability of PCF toavert UVB-induced apoptosis in thymocytes by modulating c-fos and c-junexpression, cytochrome c release, and the consequent activation of caspase-3, which were essen tial components of the UV-induced cell apoptotic pathway. Theseresults suggested that that PCF is a promising protective substance against UV radiation.