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目的探讨早期急性肺损伤(ALI)肺组织水通道蛋白5(AQP5)的变化及意义。方法 70只新生SD大鼠随机分为生理盐水对照组及内毒素(LPS)急性肺损伤组(LPS组,7个亚组)。LPS组新生大鼠腹腔注射5mg/kgLPS,各亚组分别在注射后0.5、1、2、4、8、16h和24h处死,收集肺组织行病理学观察及测肺湿/干重比值,用免疫印迹法测肺组织AQP5的表达,观察其动态变化。结果注射LPS后0.5h病理可见针尖样肺出血,随时间延长,肺出血从局灶性向弥漫性发展;注射后2、4h新生大鼠肺湿/干重比值较对照组增高(P<0.05),之后回落;注射后0.5h,肺组织AQP5表达开始下调,1～2h达到最低,与对照组比较差异有统计学意义(P<0.01),随后AQP5表达逐渐升高,8h时AQP5表达接近正常水平,8h后又出现下调直至24h,与对照组比较差异有统计学意义(P<0.01)。结论新生大鼠ALI早期即出现明显的AQP5表达下调,并与肺组织湿/干重比值呈负相关,可能是导致肺泡水肿的重要因素之一。
Objective To investigate the changes and clinical significance of aquaporin 5 (AQP5) in lungs of early acute lung injury (ALI). Methods Seventy neonatal SD rats were randomly divided into normal saline control group and LPS acute lung injury group (LPS group, 7 subgroups). The neonatal rats in LPS group were injected intraperitoneally with 5mg / kg LPS. The subgroups were sacrificed at 0.5, 1, 2, 4, 8, 16h and 24h after injection respectively. The lung tissues were collected for histopathological observation and lung wet / dry weight ratio. The expression of AQP5 in lung tissue was detected by immunoblotting and its dynamic changes were observed. Results Pulmonary hemorrhage was observed at 0.5h after LPS injection. The pulmonary hemorrhage progressed from focal to diffuse with the passage of time. The lung wet / dry weight ratio of neonatal rats at 2,4h after injection was higher than that of the control group (P <0.05) (P <0.01) .AQP5 expression gradually increased at 8h after injection, and AQP5 expression was close to normal at 8h (P <0.01). The difference was statistically significant (P <0.01) compared with the control group. Conclusions The expression of AQP5 is significantly decreased in the early stage of ALI in neonatal rats, which is negatively correlated with the ratio of wet / dry weight of lung tissue, which may be one of the important factors leading to alveolar edema.