论文部分内容阅读
芜菁花叶病毒(Turnip mosaic virus,Tu MV)是白菜类蔬菜生产上的重要病害,其中Tu MV VPg基因在Tu MV侵染白菜类蔬菜的过程中起着至关重要的作用,且不同Tu MV株系的致病性不同。本试验采用改良的重叠延伸PCR(SOE-PCR)技术,以Tu MV C4-VPg基因序列为模板,定点突变Tu MV C4-VPg与Tu MV CDN1-VPg基因间5个差异核苷酸位点(决定5个氨基酸差异)。结果表明:通过SOE-PCR技术获得了5个Tu MV C4-VPg基因的单点突变体,即Tu MV C4-VPg-Ⅰ、Tu MV C4-VPg-Ⅱ、Tu MV C4-VPg-Ⅲ、Tu MV C4-VPg-Ⅳ和Tu MV C4-VPg-Ⅴ。
Turnip mosaic virus (Tu MV) is an important disease in cabbage production. Among them, Tu MV VPg gene plays a crucial role in Tu MV infecting cabbage vegetables, and different Tu MV strains of different pathogenicity. In this study, a modified overlap extension PCR (SOE-PCR) technique was used to mutate the Tu MV C4-VPg gene sequences into five different nucleotide sites Decided to 5 amino acid differences). The results showed that five single-point mutants of Tu MV C4-VPg gene were obtained by SOE-PCR, namely Tu MV C4-VPg-Ⅰ, Tu MV C4-VPg-Ⅱ, Tu MV C4-VPg- MV C4-VPg-IV and Tu MV C4-VPg-V.