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目的:观察Er:YAG激光对根管内粪肠球菌的杀灭效果。方法:收集单直根管人离体牙130个,根管预备并高压灭菌后,接种粪肠球菌并孵育4周。将样本随机分成4组,A组:采用Er:YAG激光+52.5 g/L次氯酸钠+生理盐水+蒸馏水处理;B组:Er:YAG激光+生理盐水+蒸馏水处理;C组:52.5 g/L次氯酸钠液处理;D组:生理盐水处理。然后,采集各组处理前后根管壁表面和牙本质小管不同深度处(0～100、100～200、200～300μm)试样进行细菌培养和计数,比较各处理组的细菌减少率。结果:A、B、C 3组在根管壁不同深度处的细菌减少率均显著高于生理盐水组(P<0.05),但其中只有A组根管壁表面、牙本质小管内100μm和200μm深度处的细菌减少率为100%。结论:Er:YAG激光+52.5 g/L次氯酸钠+生理盐水+蒸馏水组对根管内粪肠球菌的杀灭效果最好。
Objective: To observe the killing effect of Er: YAG laser on Enterococcus faecalis in root canal. Methods: 130 single root canal human teeth were collected. After root canal preparation and autoclaving, Enterococcus faecalis were inoculated and incubated for 4 weeks. Group A was treated with Er: YAG laser +52.5 g / L sodium hypochlorite + saline + distilled water; Group B: Er: YAG laser + saline + distilled water; Group C: 52.5 g / L sodium hypochlorite Liquid treatment; D group: saline treatment. Then, the specimens of the root canal wall and the dentinal tubules at different depths (0-100, 100-200, 200-300μm) were collected for bacterial culture and counting before and after each treatment, and the bacterial reduction rate of each treatment group was compared. Results: The bacterial reduction rates of root canal wall at different depths in group A, B and C were significantly higher than those in saline group (P <0.05), but only in group A were 100μm and 200μm The rate of bacterial reduction at depth is 100%. Conclusion: Er: YAG laser +52.5 g / L sodium hypochlorite + saline + distilled water group had the best killing effect on Enterococcus faecalis in root canal.