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目的:研究大鼠脑内前脑啡肽原(PPE)mRNA的变化与5-HT的释放剂芬氟拉明加强针刺镇痛的关系.方法:用原位杂交组织化学法观察PPE mRNA的表达.结果:脊髓背角(Ⅰ,Ⅱ层),中缝大核,中缝背核,中央灰质,脚间核,视前外侧区,杏仁核和尾壳核内的PPEmRNA的含量大量升高(P<0.01,vs NS+EA),而在隔外侧区,视前内侧区,下丘脑腹内侧核以及脊髓背角Ⅲ—Ⅳ层的PPE mRNA的水平也有中等程度增高(P<0.05,vs NS+EA).但在丘脑却没有明显的变化.结论:PPE mRNA含量在与痛相关的区域里增高,是芬氟拉明加强针效的机制之一.
OBJECTIVE: To study the relationship between the changes of PPE mRNA and acupuncture analgesia of fenofluram with 5-HT releasing agent.Methods: The expression of PPE mRNA in rat brain was observed by in situ hybridization (P <0.05) .Conclusion: PPE mRNA in spinal dorsal horn (Ⅰ, Ⅱ), medial, dorsal raphe nucleus, central gray matter, interventricular nucleus, preoptic area, amygdala and caudate putamen increased significantly <0.01, vs NS + EA). However, there was also a moderate increase in the level of PPE mRNA in the lateral and anterior medial segments, the hypothalamic ventrolateral medulla, and the spinal dorsal horn III-IV (P <0.05, vs NS + EA), but there was no obvious change in the thalamus.Conclusion: PPE mRNA content increased in pain-related areas and is one of the mechanisms of fenfluramine to enhance needle acupuncture.