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通过观察频率、脉宽固定,电压峰值递增的脉冲电场对30只新西兰大白兔正常肝脏的杀伤效应,探讨在兔正常肝组织中建立脉冲电场分布模型的可行性,为临床电化学或电转基因治疗提供重要参考。采用重复实验及自身对照,脉冲处理3d后取材HE染色,光镜下见低脉冲剂量时两电极周围呈同心圆形坏死、界限清晰;剂量增大则同心圆形坏死区亦增大,且渐变成椭圆梭形,两电极中点处肝细胞出现核固缩、碎裂、溶解样改变,最后两椭圆梭形坏死相互重叠融合成不规则坏死,处理区尚见肝细胞的空化效应及肝组织缺血现象,实验所见与理论模拟的电场分布能较好吻合。因此,兔肝组织可作为脉冲电场生物组织杀伤效应研究中电场建模的有用载体,电脉冲在有效杀伤靶区组织的同时对周边正常组织是安全的。
By observing the killing effect of the pulsed electric field with constant pulse width and increasing voltage on the normal liver of 30 New Zealand white rabbits, the feasibility of establishing a pulsed electric field distribution model in normal rabbit liver tissues was investigated. The feasibility of electrochemiluminescence Provide important reference. Using repeated experiments and self-control, HE staining was performed after 3 days of pulse treatment. When the low pulse dose was observed under the light microscope, concentric circular necrosis was found around the two electrodes with a clear boundary; while the dose increased, the area of concentric circular necrosis increased Become oval fusiform, two electrodes at the midpoint of nuclear shrinkage, fragmentation, dissolved samples change, the last two oval spindle necrosis overlap each other into irregular necrosis, treatment area still see the cavitation effect of hepatocytes and Liver ischemia, the experimental findings and theoretical simulation of the electric field distribution can be better. Therefore, rabbit liver tissue can be used as a useful vector for electric field modeling in the study of the killing effect of pulsed electric field biological tissue. The electric pulse is safe for the surrounding normal tissue while effectively killing the target tissue.