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The purpose of this study is to find a new gene resource for the researches of molecular breeding of Rosaceae plants disease-resistance.Pyrus ussuriensis Maxim is used as a starting material to clone the full-length cDNA of NPR1(nonexpressor of pathogenesis-related genes 1) which is a key regulator in SA(salicylic acid)-mediated systemic acquired resistance(SAR) by homologous cloning and RACE techniques.The length of the cDNA sequence was 1 767 bp,the ORF was 1 761 bp,it coded 586 amino acids,pI=5.58,the relative molecular weight was 65.009 ku,contained 19 kinds of amino acids,and had full BTB/POZ and ANK domains.Compared the homology of NPR1 gene in GenBank database,the homology with Pyrus pyrifolia,Arabidopsis thaliana,Nicotiana tabacum,Lycopersicon esculentum,Oryza sativa,Helianthus annuus were 98%,62%,68%,65%,57%,63%.The homology of functional area were 99%,78%,82%,79%,74%,77%.This NPR1 gene was considered as homologic gene of Pyrus ussuriensis Maxim and named PumNPR1.
The purpose of this study is to find a new gene resource for the researches of molecular breeding of Rosaceae plants disease-resistance. Pyrus ussuriensis Maxim is used as a starting material to clone the full-length cDNA of NPR1 (nonexpressor of pathogenesis-related genes 1) which is a key regulator in SA (salicylic acid) -mediated systemic acquired resistance (SAR) by homologous cloning and RACE techniques. The length of the cDNA sequence was 1 767 bp, the ORF was 1 761 bp, it coded 586 amino acids, pI = 5.58, the relative molecular weight was 65.009 ku, contained 19 kinds of amino acids, and had full BTB / POZ and ANK domains. Compared the homology of NPR1 gene in GenBank database, the homology with Pyrus pyrifolia, Arabidopsis thaliana, Nicotiana tabacum, Lycopersicon esculentum, Oryza sativa, Helianthus annuus were 98%, 62%, 68%, 65%, 57%, 63% .The homology of functional area were 99%, 78%, 82%, 79%, 74% , 77%. This NPR1 gene was considered as a homologic gene of Pyrus ussuriensis Maxim and named PumNPR1.