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研究0.1mmol/L P和1mmol/L P下接种丛枝菌根(AM)真菌Funneliformis mosseae对沙培枳Poncirus trifoliata实生苗P吸收、转运和分泌磷酸酶的影响。结果显示,在0.1mmol/L P和1mmol/L P下,接种AM真菌都增加了枳地上部和地下部干重、一级侧根和二级侧根数,但1mmol/L P下接种AM真菌显著降低了枳主根长。与不接种AM真菌相比,1mmol/L P下接种AM真菌显著降低了枳叶片酸性磷酸酶活性,但显著增加了根系酸性磷酸酶活性及根系分泌酸性磷酸酶基因Pt PAP1的表达水平。接种AM真菌显著增加了0.1mmol/L P和1mmol/L P下叶片磷含量,根系磷含量仅在1mmol/L P下接种AM真菌后显著增加。在0.1mmol/L P和1mmol/L P下接种AM真菌显著降低了枳叶片(PtPAP1和PtPAP3)和根系(PtPAP3)分泌磷酸酶基因以及叶片磷转运蛋白基因(PtaPT5和PtaPT6)的表达水平,显著增加了枳根系PtaPT3、PtaPT5和PtaPT6的表达量。因此,推断接种AM真菌能刺激枳侧根数增多和酸性磷酸酶分泌,增强磷转运蛋白基因的表达,从而改善枳对磷养分的吸收。
The effects of Funneliformis mosseae, an AM fungi inoculated with 0.1 mmol / L P and 1 mmol / L P on P uptake, transport and secretion of phosphatase in Poncirus trifoliata seedlings were studied. The results showed that the AM fungi inoculated with AM fungi increased the dry weight, primary root and secondary root number of the upper and lower branches of the trifoliate orange at 0.1mmol / LP and 1mmol / LP, but AM fungi inoculated at 1mmol / LP significantly reduced the trifoliate orange Lord root length. AM fungi inoculated with 1 mmol / L P significantly decreased the acid phosphatase activity of leaves, but significantly increased the activity of root acid phosphatase and the expression level of the root exudate acid phosphatase gene Pt PAP1. Inoculation of AM fungi significantly increased leaf phosphorus content at 0.1 mmol / L P and 1 mmol / L P, and root phosphorus content increased significantly after inoculation of AM fungi at 1 mmol / L P. Inoculation of AM fungi at 0.1 mmol / L and 1 mmol / L significantly decreased the expression of the phosphatase gene and the PtaPT5 and PtaPT6 genes in leaves (PtPAP1 and PtPAP3) and roots (PtPAP3) The expression of PtaPT3, PtaPT5 and PtaPT6 in root of trifoliate orange. Therefore, inoculation of AM fungi inoculated with AM fungi could stimulate the increase of root number and the secretion of acid phosphatase, and enhance the expression of P transport protein, thus improving the absorption of P nutrition.