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为了建立简便、敏感、特异的方法检测病人血清乙肝病毒Dane颗粒,利用Dane颗粒外膜蛋白上的聚合人血清白蛋白受体能与人血白蛋白聚合体相结合的特性建立ELISA—步法,并与PCR检测DNA相比较。结果显示,急性乙肝检出Dane颗粒91.8％(45/49),慢性乙肝检出76.5％(72/94),大三阳血清中检出Dane颗粒95％(38/40),小三阳血中检出75.9％(60/79)。与PCR法检出DNA有较好的相关性,两法符合率87.4％(125/143)。说明用该方法检出病人血中Dane颗粒在临床上可作为判断乙肝病毒复制的重要指标。
In order to establish a simple, sensitive and specific method for the detection of serum hepatitis B virus Dane particles, the ELISA-based method was established by using the characteristics of the aggregated human serum albumin receptor on the Dane particle outer membrane protein bound to the human serum albumin polymer. And compared with PCR test DNA. The results showed that 91.8% (45/49) of Dane granules were detected in acute hepatitis B, 76.5% (72/94) in chronic hepatitis B, 95% (38/40) in Dansang serum, 75.9% (60/79) were detected. There was a good correlation between DNA and DNA detected by PCR, the coincidence rate of the two methods was 87.4% (125/143). This method can be used to detect Dane particles in the blood of patients can be used as an important indicator of hepatitis B virus replication.