1982年7月进行了系统调查,结果报告如下: 一、方法 从溪沟捕捉棘腹蛙,分别称重,去皮,取肌肉及消化道,切碎,用乳钵或碾槽分别碾成糊状,然后过筛,反复沉淀,取沉渣镜检。然后接种动物60～100天后解剖,虫体经染色制片鉴定。 July 1982 conducted a systematic survey, the results reported as follows: First, the method from the creek to catch Spinach frog, were weighed, peeled, take muscle and digestive tract, chopped, mortar or grind were respectively crushed into a paste Like, and then sieved, repeated precipitation, sediment microscopy. Then the animals were inoculated 60 to 100 days after dissection, parasites identified by staining film.