【摘 要】
:
AIM: To explore the expansion and differentiation of hepatocytoid cell induced from myeloid mesenchymal stem cell (MSC)in vitro, in order to find suitable resou
【机 构】
:
Department of Infectious Disease,Union Hospital, Tongji Medical College, Huazhong University of Scie
论文部分内容阅读
AIM: To explore the expansion and differentiation of hepatocytoid cell induced from myeloid mesenchymal stem cell (MSC)in vitro, in order to find suitable resource of hepatocytes for bioartificial liver or liver transplantation. METHODS: The rat myeloid MSC was isolated and divided into three groups which were cultured by Frieden steion method, and then were induced by culture fluid, culture fluid plus cholestatic serum and culture fluid plus hepatocyte growth factor (HGF), respectively. Hepatocytoid cell as well as expression of CK18 and AFP was observed by immunohistochemistry. RESULTS: After the induction for 21 d, hepatocytoid cell was observed, and its expression of CK18 and AFP was detected by immunohistochemistry in MSC cultured withcholestatic serum. Furthermore, on the 35th d, albumin mRNA was expressed in the cell, suggesting the inducing effect was similar to that by HGF. CONCLUSION: Rat myeloid MSC can differentiate into hepatocyte lineage under appropriate condition. This method is easy to operate.
其他文献
目的 在临床上,研究对妊娠糖尿病患者采取健康教育路径的方案进行护理后的价值.方法 选取本院妊娠糖尿病患者,例数合计为200例,选取时间范围是2017.5.5日到2018.3.5日,将其依
内镜逆行胰胆管造影术(ERCP)是目前肝胆胰疾病诊治最有效的手段,较传统的手术探查有不可比拟的优势,现已在临床广泛应用[1].但ERCP术后的感染、急性胰腺炎及高淀粉酶血症等并发症仍然是学者研究的热点.据统计,诊断性ERCP的并发症发生率约为3%,治疗性ERCP的发生率则高达6.3% ~ 11% [2-3],极大增加了患者痛苦及经济负担,甚至危及生命[4].随着内镜技术的日益成熟,内镜下鼻胆管引流
目的 总结重症患者发生垂体危象、肾上腺危象和黏液性水肿昏迷等内分泌危象的临床表现和诊疗方法.方法 回顾性分析23例发生垂体危象、肾上腺危象和黏液性水肿昏迷的重症患者的临床表现及诊疗情况.结果 主要临床表现为乏力[86.96%(20/23)],意识障碍[100.00%(23/23)],感染[100.00%(23/23)],低血压[82.61%(19/23)],低体温[43.48%(10/23)].辅
目的:研究探讨清热祛湿解毒法治疗小儿手足口病120例的临床疗效.方法:选取本院自2017年1月至2018年5月收治120例小儿手足口病患者为临床研究对象,随机分为对照组与观察组,观
目的:探讨循证护理在剖宫产护理中的应用效果.方法:针对我院2019年3月至2019年5月收治的剖宫产产妇进行临床观察护理,采用收治先后顺序将260例剖宫产产妇分成两组,针对对照组
《大学》有云:“此谓诚于中,形于外”。使人自觉那份藏在体内的美感,从而折射出外在的美。全息生态养生美容,即将所有能够激发内在美的疗法集中于一体,让被钢筋水泥困住的现代人,也
目的 探究心理护理干预配合会阴无保护接生对分娩的影响.方法 将我院产科86例待产产妇作为研究对象,入选时间为2016年6月-2017年6月,按随机数字表法分为2组,各43例;对照组产
目的研究甲泼尼龙(methylprednisolone)对大鼠视神经轴突切断术后视网膜兴奋性氨基酸转运蛋白-1(EAAT-1)和谷氨酰胺合酶(GS)表达的影响。方法建立大鼠单眼视神经轴突切断模型56只,并随机分为4组,每组14只大鼠。第1组和第2组分别给予甲泼尼龙10 mg/kg/d和20 mg/kg/d静脉注射,共5 d;第3组给予甲泼尼龙30 mg/kg静脉注射1次,以后按5.4 mg/kg/
重症急性胰腺炎(SAP)的特征是持续性器官功能衰竭,通常有一个或多个局部和(或)全身并发症,早期阶段死亡风险显著增加[1-2],并常继发感染、休克、凝血功能异常甚至弥散性血管内凝血(DIC).烟台毓璜顶医院2008年1月1日至2012年12月31日收治SAP共37例,其中4例在发病2周内出现DIC,现总结如下.病例1:男性,54岁.因“上腹痛超过20 h”入院.入院后生命体征:T 38.5℃,P