在胰岛素合成中,在A链内和A、B链间的3对二硫键正确配对是合成成功的关键。结晶牛胰岛素合成成功之后,张友尚先生、朱尚权先生等人长期进行胰岛素结构功能研究,邹承鲁先生团队进行了蛋白质折叠研究,我们在酵母中成功表达了猪胰岛素前体(porcine insulin precursor,PIP)的基因,并建立了胰岛素蛋白质工程技术。在这些研究基础上,我们于21世纪初,开展了单、双链胰岛素的体外 In insulin synthesis, the correct pairing of 3 pairs of disulfide bonds within the A chain and between the A and B chains is the key to successful synthesis. After the successful synthesis of crystalline bovine insulin, Prof. Zhang You Shang, Zhu Shangquan et al. Conducted a long-term study on insulin structural function. The Zou Chenglu team conducted a protein folding study. We successfully expressed the porcine insulin precursor (PIP) gene in yeast , And established the insulin protein engineering technology. Based on these studies, we conducted single and double-chain insulin in vitro in early 21st century