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本文比较分析了三粒小麦和普通小麦种胚萌发初期过氧化物酶活性和同工酶的变化及其对放线菌素D(AMD)和环已亚胺(CHM)的敏感性。结果表明,三粒小麦的酶活性显示单峰曲线,AMD和CHM分别抑制其酶活力的5%和24%左右。普通小麦的酶活性显示V形曲线。AMD和CHM分别抑制其酶活力的34%和67%。 两种小麦的同工酶酶谱明显不同,AMD的抑制效应均不明显,但CHM对三粒小麦的过氧化物酶同工酶抑制较强。
This article comparatively analyzed the changes of peroxidase activity and isozymes and the sensitivity to actinomycin D (AMD) and cycloheximide (CHM) in the early embryos of three wheat and common wheat. The results showed that the enzyme activity of three wheat showed a single peak curve, AMD and CHM inhibit their enzyme activity of 5% and 24%. Common wheat enzyme activity showed a V-shaped curve. AMD and CHM inhibited 34% and 67% of their enzyme activity, respectively. The isoenzyme zymograms of the two wheat varieties were significantly different, the inhibitory effect of AMD was not obvious, but CHM on the three wheat peroxidase isoenzyme inhibition strong.