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目的：探讨ｒａｓ、ｎｅｕ、ｐ５３基因在肿瘤演进过程中的作用。方法：采用链蛋白生物素免疫组化法（ＬＳＡＢ）检测了ｒａｓ、ｎｅｕ、ｐ５３基因产物ｐ２１、ｐ１８５、ｐ５３蛋白在卵巢浆液肿瘤中的表达情况。结果：三种蛋白在良性、交界性和恶性肿瘤组织中阳性率呈递增趋势，联合表达率亦呈递增趋势，差异显著（Ｐ＜０．０５），ｐ２１表达与患者生存期有关，过度表达组低于非过度表达组（Ｐ＜０．０１）。结论：联合检测ｐ２１、ｐ１８５、ｐ５３蛋白在卵巢浆液性肿瘤组织中的表达情况，对该肿瘤的诊断及预后判定具有重要参考价值
Objective: To investigate the role of ras, neu and p53 genes in tumor progression. METHODS: The expression of ras, neu, p53 gene products p21, p185, p53 protein in ovarian serous tumors was detected by streptavidin-biotin immunohistochemistry (LSAB). Results: The positive rates of three proteins in benign, borderline, and malignant tumors showed an increasing trend, and the combined expression rate also showed an increasing trend. The difference was significant (P<0.05). The expression of p21 was related to the survival period of patients. Overexpression group Less than non-overexpression group (P <0.01). Conclusion: The combined detection of p21, p185 and p53 proteins in ovarian serous tumor tissues has important reference value for the diagnosis and prognosis of the tumor.