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In this paper it is demonstrated that (~3H)2’-5’P_3A_3 can bind to the peritoneal macrophages from Wistar rat. This binding is strongly inhibited by cold 2’-5’P_3A_3 while the inhibiting capacity of 2’-5’P_3I_3 and 2’-5’A_3 is very small, showing that this binding is highly specific. And the binding of (~3H)2’-5’P_3A_3 is reversible, saturable and with high affinity. A Scatchard analysis of the binding data gives a linear plot, an apparent dissociation constant (Kd) about 1.3×10~(-7) M and binding sites per cell about 2.1×10~7. The above evidence shows the existence of the 2’-5’P_3A_3 receptor, a fact which, to our knowledge, has not been reported before.
This binding is strongly inhibited by cold 2’-5’P_3A_3 while the inhibiting capacity of 2’-5 ’ P_3I_3 and 2’-5’A_3 is very small, showing that this binding is highly specific. And The binding of (~ 3H) 2’-5’P_3A_3 is reversible, saturable and with high affinity. A Scatchard analysis of the binding data gives a linear plot of an apparent dissociation constant (Kd) of about 1.3 × 10 -7 M and binding sites per cell about 2.1 × 10 -7. The above evidence shows the existence of the 2’-5’P_3A_3 receptor, a fact which, to our knowledge, has not been reported before.