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人工合成ＨＩＬ－４ｃＤＮＡ５’末端３０个碱基序列作为探针，并将其与建立的基因文库进行克隆杂交，获得ＨＩＬ－４ｃＤＮＡ片段。将该片段插入πＨ３Ｍ质粒并在猴ＣＯＳ细胞获得表达，再从质粒ｐｄＫＣＲ和ｐｄＢＰＶ－１出发构建了新的表达质粒ｐｄＢＨ－ｈＩＬ－４，经磷酸钙沉淀法导入小鼠Ｃｌ２７细胞获得成功转化的细胞克隆，该克隆细胞可高效地分泌ＩＬ－４活性多肽。改进细胞培养方法，有利于产物的提取和纯化；另外，应用测定Ｂ细胞表面Ｆｃε受体表达的原理和方法测定ＩＬ－４生物学活性更具特异性。
HIL-4 cDNA was synthesized by using the 30 base sequence of 5’-end of HIL-4 cDNA as a probe and cloning hybridization with the established gene library. The fragment was inserted into the πH3M plasmid and expressed in the monkey COS cells. Then, a new expression plasmid pdBH-hIL-4 was constructed from the plasmids pdKCR and pdBPV-1 and introduced into the mouse Cl27 cells by calcium phosphate precipitation to obtain the successfully transformed cells Clone, the clonal cells can be highly efficient secretion of IL-4 polypeptide. Improve cell culture method is conducive to the extraction and purification of products; In addition, the determination of B cell surface Fcε receptor expression of the principle and method to determine IL-4 biological activity more specific.