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[Objective]To optimize the extraction technology of polysaccharides from the leaves of Coix lacryma-jobi L.by cellulase method.[Methods]Effects of pH value,cellulase dosage,extraction time and hydrolysis temperature on the extraction rate of polysaccharides were researched by single factor test and orthogonal test.[Results]The optimal extraction technology of polysaccharides in C.lacryma-jobi leaves was as follows:was 5 pH,50℃hydrolysis temperature,2.5 h extraction time,and 500U cellulase in 1 g powder of C.lacryma-jobi leaves.Under this extraction technology,the average extraction rate of polysaccharides was 14.45%and RSD was 0.18%.[Conclusions]Extracting the polysaccharides from C.lacryma-jobi leaves by cellulase method had the advantages of time saving and high efficiency.