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目的了解新疆库车县维吾尔族人群HIV-1和HCV流行株的亚型分布情况。方法检测库车县70份HIV-1抗体阳性的血浆标本的HCV抗体,并抽提血浆病毒RNA,反转录后扩增HIV-1 gag基因和构建进化树并分别与HIV-1和HCV国际标准株比较,确定被检标本的基因分型。结果 70例HIV-1感染者中,检出HCV抗体阳性67例、占95.7%;从异性性接触者中检出HCV抗体阳性15例、占21.4%,从静脉注射吸毒者中检出HCV抗体阳性55例、占78.6%;67例成功扩增HIV-1 gag基因序列,均为CRF 07_BC亚型;67例成功扩增HCV 5’UTR基因片段,分为4个亚型,以1b型为主占36.8%。结论新疆库车县HIV-1感染者特别是静脉注射吸毒者中HCV合并感染率较高,HIV-1以CRF 07_BC亚型为主要流行毒株,HCV则以1b和3a亚型为主要流行毒株。
Objective To understand the subtype distribution of HIV-1 and HCV epidemic strains in Uighur population in Kuqa County of Xinjiang Autonomous Region. Methods The anti-HCV antibodies of 70 HIV-1 positive plasma samples from Kuqa County were detected and the plasma viral RNA was extracted. After reverse transcription, HIV-1 gag gene was amplified and the phylogenetic tree was constructed. Standard strains compared to determine the type of test specimens. Results Of the 70 cases of HIV-1 infected persons, 67 cases were positive for HCV antibodies, accounting for 95.7%. 15 cases (21.4%) were positive for HCV antibodies from heterosexual contacts, and HCV antibodies were detected from intravenous drug users Positive in 55 cases, accounting for 78.6%; 67 cases successfully amplified HIV-1 gag gene sequence, all CRF 07_BC subtypes; 67 cases of HCV 5’UTR gene fragment amplified successfully, divided into 4 subtypes to 1b type The Lord accounted for 36.8%. Conclusions The prevalence of HCV co-infection among HIV-1 infected persons, especially intravenous drug users in Kuqa County of Xinjiang Autonomous Region is high. HIV-1 strain CRF 07_BC is the predominant epidemic strain and HCV strains 1b and 3a are the major epidemic strains Strain.