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本研究采用聚丙烯酰胺凝胶电泳和细胞体外培养方法,观察酶消化后人羊膜基膜的成分,以及它能否支持鸡胚前脑神经细胞的生长。肝素酶Ⅱ消化后的人羊膜基膜,对前脑神经元生长的支持作用,比未消化的基膜增强150％。电泳分析的结果表明,肝素酶Ⅱ消化后,类胶原多肽2α_1(260kD)和2α_2(225kD)减少,类基板蛋白多肽(212kD和200kD)增多;而经肝素酶Ⅰ、胶原酶Ⅰ消化后,基板蛋白减少。本文提示,基板蛋白是人羊膜基膜中促鸡胚前脑神经细胞生长的主要成分。
In this study, polyacrylamide gel electrophoresis and in vitro cell culture methods were used to observe the composition of human amniotic membrane after enzymatic digestion and whether it can support the growth of neural cells in chicken embryo forebrain. Human amniotic membrane after heparinase digestion supported the growth of forebrain neurons more than 150% of the undigested basilar membrane. The results of electrophoresis showed that the digestible collagen peptides 2α_1 (260kD) and 2α_2 (225kD) decreased after heparanase II digestion, and the substrates of the proteoglycan polypeptides (212kD and 200kD) increased. After digestion with heparanase Ⅰ and collagenase Ⅰ , Reduced substrate protein. This article suggests that the substrate protein is the main component of human amniotic membrane-based membrane to promote the growth of chicken embryonic forebrain neurons.