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目的:为了明确宁夏隆德县黄芪根瘤种类及结瘤情况。方法:采用田间调查方法对宁夏中药材种植基地黄芪根瘤菌进行了调查。结果:隆德县黄芪根瘤形态有掌状、棒状、球形,以掌状为主占59%,棒状、球形各占31%、10%;颜色有黄色、粉白色,以黄色为主占89%,粉白色占11%;大小2~7 mm;结瘤部位以侧根和须根为主。不同地块根瘤形态不同,三星村以棒状为主占85%,赵楼村、庞庄村、观庄村以掌状为主各占56%、52%、82%。根瘤数量呈季节性变化,黄芪萌动期开始结瘤,6~7月份生长期结瘤量达到高峰,10月份收获期根瘤数量减少。不同形态的根瘤经革兰氏染色均呈G-;温度<4℃、>45℃不能生长;pH≤5、≥12不能生长;NaCl浓度≥15%不能生长。结论:调查结果可为筛选具有特殊抗逆性的黄芪根瘤菌菌株提供种质资源和理论依据。
Objective: To clarify the species and nodulation of Astragalus root nodule in Lund county of Ningxia. Methods: Field investigation was used to investigate the rhizobia of Astragalus in Chinese traditional medicine planting base in Ningxia. Results: The root nodules of Astragalus in Luodeng County were palm-shaped, rod-shaped and spherical, accounting for 59% with palmate shape and 31% with spire and spherical shape respectively. The color of the root was yellow and pink with 89% , Powder white accounted for 11%; size 2 ~ 7 mm; nodules to the lateral roots and fibrous roots. The root nodules of different plots are different in shape, with 85% of the sticks in the village of Samsung, while 56%, 52% and 82% of the palm trees in Zhaolou Village, Pangzhuang Village and Guanzhuang Village, respectively. The number of nodules showed a seasonal change, nodulation started during the germination period of Astragalus, and the nodulation amount reached the peak during the growth period from June to July. The number of nodules at harvest decreased in October. Gram stains of different forms of nodules were G-; temperature <4 ℃,> 45 ℃ can not grow; pH ≤ 5, ≥ 12 can not grow; NaCl concentration ≥ 15% can not grow. Conclusion: The results can provide germplasm resources and theoretical basis for the screening of Rhizobium strains with special resistance.