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CMLs蛋白是一类具有Ca2+结合的EF-hand保守结构域蛋白,广泛参与钙依赖的信号传导途径,在植物生长发育及生物胁迫与非生物胁迫响应中起着重要的作用。通过NCBI和植物基因组注释数据库等筛选并获得了68个大豆GmCML蛋白;分析了所有蛋白的基本特性;通过与拟南芥CMLs基因的进化树分析表明,GmCML家族基因属于8个亚类;对GmCML家族基因进行了染色体定位与重复基因进化关系分析,发现其具有较高的进化速率;序列比对发现GmCML类蛋白含有2~4个保守的EF-hand结构域;利用芯片数据对野生大豆在碱胁迫下的叶和根中的CMLs基因表达模式进行了分析,得到26个GsCML基因在碱胁迫下有显著的差异表达,且在根和叶中的差异表达模式不同,表明这些基因参与植物碱胁迫应答,且具组织表达特异性。
CMLs proteins are a class of Ca2 + -conjugated EF-hand conserved domain proteins that are widely involved in calcium-dependent signaling pathways and play an important role in plant growth and development as well as biotic and abiotic stress responses. Sixty-four soybean GmCML proteins were screened and obtained from NCBI and plant genome annotation databases. The basic characteristics of all proteins were analyzed. The phylogenetic tree analysis of CMLs in Arabidopsis showed that the GmCML family of genes belong to eight subgroups. The results showed that GmCML-like proteins contained 2-4 conserved EF-hand domains. Sequence analysis showed that GmCML-like proteins contained two or four conserved EF-hand domains. Under stress, the expression patterns of CMLs genes in leaves and roots were analyzed, and 26 GsCML genes were significantly differentially expressed under alkaline stress, and the differential expression patterns in roots and leaves were different, indicating that these genes are involved in plant alkali stress Response, and with tissue expression specificity.