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人类体液中的3β12α—二羟—5—胆酸(3β12α-Δ~5)在肝胆疾病中的改变目前几乎无人知晓。作者用肝胆病患者的尿标本,检出3β12α-Δ~5,并对不饱和胆汁酸3β12α-Δ~5和3β—二羟(3β-Δ~5)进行定量分析,探讨如下。对象与方法:正常人7例,肝胆疾病30例(代偿性肝硬变10例,失代偿性肝硬变11例,阻塞性黄疸9例)。取尿标本5～10ml,加入LCA-d_4、DCA-d_5、CDCA-d_4、UDCA-d_4、CA-d_3、3β-Δ~5-d_8,按柳沢等(Anal Biochem 104:75,1980)的方法制备乙酯二甲基乙基硅烷醚,用气相层析法质量分析计对3β_(12)α-Δ~5经DCA-d_5做定量分析。结果与讨论:①尿中不饱和胆汁酸:3β12α-Δ~5在
The changes of 3β12α-dihydroxy-5-cholic acid (3β12α-Δ5) in human body fluid in hepatobiliary diseases are almost unknown at present. The authors used urine samples of patients with hepatobiliary disease to detect 3β12α-Δ5, and quantitatively analyzed 3β12α-Δ5 and 3β-dihydroxy (3β-Δ5) of unsaturated bile acids as follows. Subjects and Methods: Normal in 7 cases, liver and gallbladder disease in 30 cases (compensatory cirrhosis in 10 cases, decompensated cirrhosis in 11 cases, obstructive jaundice in 9 cases). Urine specimens were collected at 5 to 10 ml, and LCA-d_4, DCA-d_5, CDCA-d_4, UDCA-d_4 and CA-d_3,3β-Δ5-d_8 were added and the urine samples were analyzed by the method of Anal Biochem 104: Ethyl dimethylsilyl ether was prepared. Quantitative analysis of 3β 12 -α-Δ 5 by DCA-d 5 was performed by gas chromatography. Results and discussion: ① urinary unsaturated bile acids: 3β12α-Δ ~ 5 in