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目的观察大肠癌患者外周血CD4~+ CD25~+调节性T(Treg)细胞数量的变化,探讨其与肿瘤临床病理特征的关系。方法应用流式细胞仪分析大肠癌患者和健康对照组外周血单个核细胞的Treg细胞数目,比较Treg细胞百分比变化与大肠癌临床病理特征的相关性。结果结直肠癌患者Treg细胞占CD4~+T细胞百分比(15±8)%,与健康对照组(6.0±3.5)比较,差异有统计学意义(P<0.01);CEA正常的结直肠癌患者Treg细胞占CD4~+T细胞百分比(13±4)%,与CEA升高的结直肠癌患者Treg细胞(17±10)%比较,差异有统计学意义(P<0.05);Duke’s C和D期的结直肠癌患者Treg细胞占CD4~+T细胞百分比(19±6)%,与A期和B期Treg细胞百分比(12±5)%比较,差异有统计学意义(P<0.05)。结直肠癌患者外周血TGF-β1含量(1.2±0.4)ng/L,与Treg细胞数目呈正相关(r=0.253,P<0.05)。结论Treg细胞在大肠癌患者中比例升高,可能是产生肿瘤免疫抑制的重要机制,Treg细胞增加可作为大肠癌患者肿瘤进展的重要标志。
Objective To observe the changes of CD4 ~ + CD25 ~ + regulatory T (Treg) cells in peripheral blood of patients with colorectal cancer and to explore its relationship with clinicopathological features. Methods The number of Treg cells in peripheral blood mononuclear cells of patients with colorectal cancer and healthy controls was analyzed by flow cytometry. The correlation between the percentage of Treg cells and clinicopathological features of colorectal cancer was analyzed. Results The percentage of Treg cells in CD4 ~ + T cells was (15 ± 8)% in colorectal cancer patients, which was significantly lower than that in healthy controls (6.0 ± 3.5) (P <0.01). The patients with normal CEA The percentage of Treg cells in CD4 ~ + T cells (13 ± 4)% was significantly higher than that in Treg cells (17 ± 10)% in patients with elevated CEA, P <0.05; Duke’s C and D The percentage of Treg cells in CD4 ~ + T cells (19 ± 6%) was significantly higher than that in A and B phases (12 ± 5%) (P <0.05). The level of TGF-β1 in peripheral blood of patients with colorectal cancer (1.2 ± 0.4) ng / L was positively correlated with the number of Treg cells (r = 0.253, P <0.05). Conclusion Treg cells in colorectal cancer patients may be an important mechanism of tumor immunosuppression. The increase of Treg cells can be used as an important marker of tumor progression in patients with colorectal cancer.