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目的:建立毛细管气相色谱法同时测定醒脑静注射液中麝香酮、龙脑、樟脑、异龙脑的含量。方法:采用气相色谱法,DB-FFAP(0.1μm×0.53 mm×30 m)毛细管色谱柱分离测定,FID检测器。进样口温度230℃。检测器温度240℃。用氮气作为载气,流速2.5 mL·min-1;色谱柱采用梯度升温,开始温度85℃,以5℃·min-1的升温速率升至185℃,以20℃·min-1的升温速率升至215℃,以1℃·min-1的升温速率升至230℃,保持5 min。分流比15∶1,进样量1μL,外标法定量。结果:麝香酮、龙脑、樟脑、异龙脑在同一色谱条件下获得分离,麝香酮、龙脑、樟脑、异龙脑在测定范围内呈良好线性关系,回收率符合要求。结论:方法简便、灵敏、准确,分离度、重复性好。
Objective: To establish a simultaneous determination of muscone, borneol, camphor and isoborneol in Xingnaojing Injection by capillary gas chromatography. Methods: The chromatographic separation was performed on a capillary column with DB-FFAP (0.1 μm × 0.53 mm × 30 m) and FID detector by gas chromatography. Inlet temperature 230 ℃. Detector temperature 240 ℃. The flow rate was 2.5 mL · min-1 with nitrogen as the carrier gas. The temperature of the column was increased by 85 ℃ and the temperature was raised to 185 ℃ with the temperature rising rate of 5 ℃ · min-1. The temperature was raised at 20 ℃ · min-1 Rose to 215 ° C and increased to 230 ° C at a heating rate of 1 ° C · min-1 for 5 minutes. Shunt than 15: 1, injection volume 1μL, external standard method. Results: Muscone, borneol, camphor and isoborneol were separated under the same chromatographic conditions. Musk ketone, borneol, camphor and isoborneol showed a good linear relationship in the determination range, and the recovery rate met the requirements. Conclusion: The method is simple, sensitive, accurate, accurate and reproducible.