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背景:作为骨折发生的重要临床预测因子,骨密度在一定程度上由遗传因素决定。护骨素基因是骨质疏松症发病中的重要候选基因。目的:探讨护骨素基因T245G多态性与骨密度的相关性。方法:选取2008-09/2010-04在北京大学人民医院进行常规查体的老年人281名,其中男182名,女99名。应用PCR-RFLP结合DNA测序检测护骨素基因T245G多态性,使用双能X射线骨密度测量仪测定受试者腰椎、髋部标准位置及前臂的骨密度。同时收集受试者的生化指标及临床观察项目。应用ANOVA方法分析护骨素基因T245G多态性与各检测指标的关系。结果与结论:在老年男性及绝经后女性中,T245G基因T,G等位基因频率分布差异无显著性意义(P>0.05)。在老年男性中,GG和TG基因型具有较高的腰椎骨密度,而TT基因型的腰椎骨密度较低(P<0.05),Ward’s三角区及前臂骨密度在各基因型间差异无显著性意义(P>0.05)。在绝经后女性中,T245G多态性与骨密度无关,说明护骨素基因与老年男性腰椎骨密度有关。
Background. As an important clinical predictor of fracture, bone mineral density is to some extent determined by genetic factors. Osteoprotegerin gene is an important candidate in the pathogenesis of osteoporosis. Objective: To investigate the relationship between T245G polymorphism of the osteoprotegerin gene and bone mineral density. Methods: A total of 281 elderly people were enrolled in Peking University People’s Hospital from September 2008 to April 2010, including 182 males and 99 females. The T245G polymorphism of the osteoprotegerin gene was detected by PCR-RFLP combined with DNA sequencing. The lumbar spine and hip position and the forearm bone mineral density were measured by dual-energy X-ray absorptiometry. At the same time to collect subjects biochemical indicators and clinical observation items. The ANOVA method was used to analyze the relationship between the T245G polymorphism of osteoprotegerin gene and various test indexes. RESULTS AND CONCLUSION: In elderly men and postmenopausal women, there was no significant difference in the frequency distribution of T and G alleles (P> 0.05). In elderly men, GG and TG genotypes had higher lumbar BMD, while TT genotype had lower lumbar BMD (P <0.05). There was no significant difference in Ward’s trigone and forearm BMD between genotypes Significance (P> 0.05). In postmenopausal women, T245G polymorphism has nothing to do with bone mineral density, indicating that osteoprotegerin gene and lumbar spine bone density in elderly men.