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目的:运用灰色关联度法分析影响杜仲皮和叶中4种有效成分含量产地差异性的主要环境因子。方法:利用HPLC同时测定10个产地10年生杜仲皮和叶中桃叶珊瑚苷、京尼平苷酸、绿原酸、松脂醇二葡萄糖苷的含量,流动相分别为乙腈(A)-0.1%磷酸水(B)梯度洗脱(0~25 min,5%~13%A;25~45 min,13%~15%A;45~80 min,15%~21%A),流动相乙腈(A)-0.1%磷酸水(B)梯度洗脱(0~20 min,5%~12%A;20~35 min,12%~13%A;35~45 min,13%~19%A;45~65min,19%~25%A),检测波长均为210 nm。检测杜仲生长地土壤的全氮、全磷、全钾、碱解氮、速效磷、速效钾、有机质及p H,获得土壤因子数据,运用灰色关联度法对4种有效成分与气候及土壤因子的相关性进行分析。结果:10个产地同一生长年限杜仲皮、叶中4种有效成分的含量存在较大差异。碱解氮、速效钾、有机质、年平均相对湿度、年平均最高气温对4种成分在杜仲不同组织中的影响较大。结论:杜仲不同组织部位中4种有效成分含量与环境因子的灰色关联度分析明确了主导环境因子,为合理施肥及环境调控来提高杜仲次生代谢产物的含量提供参考,为杜仲的适宜种植区选择及其道地性产生原因分析提供实验依据。
OBJECTIVE: To analyze the main environmental factors influencing the origin difference of the content of four active ingredients in the leaves and leaves of Eucommia bark by using the gray relational degree method. Methods: The contents of aucubin, geniposidic acid, chlorogenic acid and pinoresinol diglucoside in 10-year-old ten-year-old Eucommia bark and leaves were determined by HPLC. The mobile phases were acetonitrile-A (B) gradient elution (0-25 min, 5-13% A; 25-45 min, 13-15% A; 45-80 min, 15-21% A) 0.1% phosphoric acid water (B) gradient elution (0-20 min, 5-12% A; 20-35 min, 12-13% A; 35-45 min, 13-19% A; 65min, 19% ~ 25% A), the detection wavelength is 210 nm. Soil total nitrogen, total phosphorus, total potassium, alkaline hydrolysis nitrogen, available phosphorus, available potassium, organic matter and p H were measured in soil of Eucommia ulmoides growing. The soil factor data were obtained and the four effective components were analyzed with gray relational method and climatic and soil factors The relevance of the analysis. Results: The contents of four effective components in Eucommia bark and leaves of the same place of production in the same growth period differed greatly. The contents of alkaline nitrogen, available potassium, organic matter, annual average relative humidity and annual mean maximum temperature greatly influenced the four components in different tissues of Eucommia. CONCLUSION: Gray relational analysis of the content of four active components in different tissue parts of Eucommia ulmoides with environmental factors clearly dominates the environmental factors and provides a reference for improving the secondary metabolites content of Eucommia ulmoides, which is the suitable planting area for Eucommia ulmoides Choice and its origin to provide the experimental basis for analysis.