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建立了HPLC法测定蛹虫草子实体中甘露醇含量的方法。通过比较提取溶剂、提取方式及提取时间等条件对甘露醇提取效果的影响,确定甘露醇分析的前处理方法为:1g子实体粉中加入150 mL 90%乙醇热回流提取1h。采用SUGAR SP0810柱(300 mm×8 mm)为分析柱,超纯水为流动相,流速1.0 mL/min,柱温70℃,示差折光检测器检测,进样量10μL。甘露醇在0.04-9.9 mg/mL范围内线性关系良好,回归方程为y=103860x+2183.9(r=0.9999),平均回收率为104.27%,RSD=2.19%(n=9)。本方法准确度高,稳定性、精密度、重现性好,适用于蛹虫草子实体中甘露醇含量的分析。
A method for the determination of mannitol in Cordyceps militaris by HPLC was established. The effects of extraction solvent, extraction method and extraction time on the extraction of mannitol were compared. The pretreatment method of mannitol analysis was as follows: 150 mL of 90% ethanol was added into 1 g fruiting body powder for 1 h by hot reflux extraction. Using SUGAR SP0810 column (300 mm × 8 mm) as analytical column, ultrapure water as mobile phase, the flow rate of 1.0 mL / min, the column temperature of 70 ℃, refractive index detector detection, injection volume 10μL. The linearity of mannitol in the range of 0.04-9.9 mg / mL was good. The regression equation was y = 103860x + 2183.9 (r = 0.9999). The average recovery was 104.27% and RSD was 2.19% (n = 9). The method has high accuracy, stability, precision and reproducibility, and is suitable for the analysis of mannitol content in Cordyceps militaris fruiting body.