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目的:探讨葛根素对大鼠急性脑缺血损伤及缺血再灌注损伤后HSP70表达的影响。方法:采用夹闭大脑中动脉造成局部脑缺血模型,夹闭大脑中动脉后再通造成局部脑缺血再灌注模型,对脑组织用免疫组化SP法作HSP70染色,观察其HSP70表达情况。结果:大鼠急性脑缺血损伤后葛根素干预实验组的HSP70表达强度明显强于单纯缺血实验组(P<0.01);大鼠脑缺血再灌注损伤后葛根素保护组实验侧HSP70表达强度明显强于非保护组实验侧(P<0.01)。结论:葛根素有上调大鼠急性脑缺血及缺血再灌注损伤后HSP70表达的作用。
Objective: To investigate the effect of puerarin on the expression of HSP70 in acute cerebral ischemic injury and ischemia-reperfusion injury in rats. METHODS: The model of focal cerebral ischemia caused by middle cerebral artery occlusion was used. After the middle cerebral artery was occluded, the cerebral ischemia-reperfusion model was established. The brain tissue was stained by SP immunohistochemical method and HSP70 expression was observed. . RESULTS: The expression of HSP70 in rats treated with puerarin after acute cerebral ischemic injury was significantly stronger than that in the ischemic group alone (P<0.01); the expression of HSP70 in the experimental side of puerarin-protected rats after cerebral ischemia reperfusion injury. The intensity was significantly stronger than that of the non-protected group (P<0.01). CONCLUSION: Puerarin can up-regulate the expression of HSP70 after acute cerebral ischemia and ischemia-reperfusion injury in rats.