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付溶血性弧菌(Vibrio parahaemolyticus)是水产品中的一种重要食物中毒菌,它在沿岸海水和海产动物体上都有广泛的分布,调查了青岛近岸海水和几种常见水产品污染了该菌的情况。在检测方法上,修定了一种选择性较强的培养基(SAC)。实验效果表明,SAC较其他常用的几种培养对付溶血性弧菌检出的阳性率和灵敏性较高,与普遍应用的TCBS法比较,X~2=4.28.P<9.95,效果有显著意义。青岛近岸海水中付溶血性弧菌的平均值为63.25—463cfu/100mL,季节性和海区对其检出率有明显影响。夏季检出率最高,冬季最低。在几种水产品中,以贝类含量最高(6900cfu/100g)。对检出的菌株,选出部分作了主要生理、生化特性的鉴定,证实有85％以上的株数符合付溶血弧菌的特征,并初步实验证明,这是一种温血动物和冷血动物的共患菌。
Vibrio parahaemolyticus, an important food poisoning agent in aquatic products, has a wide distribution in coastal and marine animals. It has investigated the pollution of coastal waters and several common aquatic products in Qingdao The case of the bacteria. In the detection method, modified a more selective medium (SAC). The experimental results showed that SAC had a higher positive rate and higher sensitivity to Vibrio parahaemolyticus than other commonly used cultures. Compared with the commonly used TCBS method, X 2 = 4.28.P <9.95, the effect was significant . The average value of Vibrio parahaemolyticus in coastal seawater of Qingdao was 63.25-463cfu / 100mL, and seasonal and sea area had a significant impact on the detection rate. The highest detection rate in summer, the lowest in winter. In several aquatic products, the highest content of shellfish (6900cfu / 100g). The strains of the test, the selected part of the main physiological and biochemical characteristics of the identification, confirmed that more than 85% of the number of strains in line with the characteristics of Vibrio parahaemolyticus, and preliminary experiments show that this is a warm-blooded and cold-blooded animals Co-infection.