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目的:研究白细胞介素-6(IL-6)基因启动子MbiI多态性在子宫内膜异位症、子宫肌瘤患者及正常人中的分布。方法:应用聚合酶链反应-限制性片段长度多态性技术检测98名子宫内膜异位症患者与97名子宫肌瘤患者及99名健康成年女性的白细胞介素-6基因启动子MbiI多态性,比较三组白细胞介素-6基因型和等位基因的分布频率。结果:CC、CG和GG基因型在子宫内膜异位症患者中分别为55.10%、42.86%、2.04%;在子宫肌瘤患者中分别为71.13%、27.84%、1.03%;在健康成年女性中分别为70.71%、28.28%、1.01%。子宫内膜异位症组与子宫肌瘤组及健康成年女性组基因型的分布频率差异有显著性,而子宫肌瘤组与健康成年女性组无显著性差异。结论:白细胞介素-6基因启动子MbiI的多态性分布与子宫内膜异位症的发病具有一定程度的相关性,而与子宫肌瘤的发病无相关性。
Objective: To investigate the distribution of MbiI polymorphism of interleukin-6 (IL-6) gene promoter in endometriosis, uterine fibroid patients and normal subjects. Methods: Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) was used to detect the MbiI promoter of 98 patients with endometriosis and 97 patients with uterine fibroids and 99 healthy adult women The distribution of interleukin-6 genotypes and alleles in three groups were compared. Results: The genotypes of CC, CG and GG were 55.10%, 42.86% and 2.04% in patients with endometriosis, 71.13%, 27.84% and 1.03% in patients with uterine fibroids, respectively. In healthy adult women Respectively, 70.71%, 28.28%, 1.01%. There was a significant difference in genotype distribution between endometriosis group and uterine fibroid group and healthy adult female group, but there was no significant difference between uterine fibroid group and healthy adult female group. Conclusion: The polymorphism distribution of interleukin-6 gene promoter MbiI has some correlation with the pathogenesis of endometriosis, but not with the incidence of uterine fibroids.