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以猴耳环成熟的种胚无菌萌发小苗为外植体,研究不同种类及浓度的植物生长物质对丛芽诱导增殖和植株再生的影响,建立完整的离体快繁体系。结果表明,猴耳环种胚最佳灭菌方式为0.1% HgCl_2处理12 min,污染率16.10%,无菌萌发率78.66%;丛芽初代诱导最佳培养基为MS+6-BA 0.5 mg·L~(-1),诱导率100%;继代增殖最适宜的培养基为MS+6-BA 0.7 mg·L~(-1)+NAA 0.05 mg·L~(-1)+IBA 0.3 mg·L~(-1),增殖系数3.95;1/2MS+NAA 0.1 mg·L~(-1)+活性炭0.1 g·L~(-1)适合诱导生根,45 d后可获得健壮植株,生根率100%,平均根数4.41。将生长良好的植株移栽到等体积比蛭石、沙子、泥炭土混合的基质中,成活率66.67%。
Mature seed germination of monkey earrings as explants to study the different types and concentrations of plant growth substances on buds induced proliferation and plant regeneration, the establishment of a complete in vitro propagation system. The results showed that the optimal sterilization method for monkey earring embryos was 0.1% HgCl 2 for 12 min, with a contamination rate of 16.10% and a germ-free germination rate of 78.66%. The best culture medium for initial bud formation was MS + 6-BA 0.5 mg · L ~ (-1), and the induction rate was 100%. The most suitable medium for subculture was MS + 6-BA 0.7 mg · L -1 NAA 0.05 mg · L -1 IBA 0.3 mg · L -1 and propagation coefficient 3.95; 1/2 MS + NAA 0.1 mg · L -1 and activated carbon 0.1 g · L -1 were suitable for inducing rooting, and robust plants could be obtained after 45 days. The rooting rate 100%, the average number of 4.41. Transplanted plants with good growth into the medium of vermiculite, sand and peat mixed with the same volume, the survival rate was 66.67%.