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目的 检测多发性硬化 (MS)患者外周血单个核细胞在地塞米松 (Dex)影响下的 IFN- γ和 IL- 10的分泌细胞水平。方法 采用酶联免疫斑点技术 (EL ISPOT)检测体外培养的外周血单个核细胞 (MNC)在 CNS髓鞘素抗原 MBP刺激下的地塞米松对照试验 ,检测 IFN -γ和 IL - 10分泌性 T细胞水平 ,并与其他神经疾病 (OND)组及健康对照组的检测结果进行对比。结果 显示 MS患者 IFN- γ分泌细胞水平高于对照组 ,Dex使 MS患者 IFN- γ分泌细胞减少 ,对 IL- 10分泌细胞无明显影响。结论 MS患者存在 Th1/ Th2细胞因子的失衡 ,Dex能抑制 MS Th1类细胞因子 IFN- γ,其治疗作用可能与此有关。
Objective To detect the levels of IFN-γ and IL-10 secreted by peripheral blood mononuclear cells in patients with multiple sclerosis (MS) under the influence of Dex. Methods ELISPOT was used to detect the dexamethasone control experiment of in vitro cultured peripheral blood mononuclear cells (MNCs) stimulated by MBP of CNS myelin antigen. The secretion of IFN - γ and IL - 10 Cell level, and with other neurological disease (OND) group and healthy control group test results were compared. The results showed that the level of IFN-γ secreting cells in MS patients was higher than that in control group. Dex decreased the number of IFN-γ secreting cells in MS patients and had no significant effect on IL-10 secreting cells. Conclusion There is an imbalance of Th1 / Th2 cytokines in MS patients. Dex can inhibit the IFN-γ of MS Th1 cytokines and its therapeutic effect may be related to this.