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目的通过对三组不同人群尿样和血样的分析比较,寻找三氯乙烯对人体免疫系统造成的损伤特异性指标。方法收集三氯乙烯接触工人、非接触有机溶剂行业工人、三氯乙烯患者的血样和尿样,用顶空-气相色谱法测定尿样中三氯乙酸,用免疫球蛋白测定试剂盒测定血样中IgG、IgA、IgM、IgE和外周血T淋巴细胞亚群,用分光度法测定血清中超氧化物歧化酶(SOD)活力、血清中丙二醛(MDA)含量、血清中谷胱甘肽过氧化物酶(GSH-PX)活力。结果 IgA、IgE和IgG水平无明显改变,但观察组和比例组的IgM比对照组低,有显著性差异。尿中三氯乙酸观察组及病例组的三氯乙酸比对照组高,有显著性差异。病例组和观察组CD3+、CD4+、CD4+/CD8+均低于对照组,CD8+高于对照组,各组均有显著性差异。观察组及病例组与对照组比较,MDA、SOD和GSH-PX水平明显升高,差异有统计学意义。结论接触三氯乙烯会改变机体的免疫系统,体内脂质过氧化作用会增强。
Objective To analyze and compare the urinary samples and blood samples of three different groups to find the trichlorethylene damage-specific indicators of the human immune system. Methods Blood samples and urine samples from trichlorethylene exposure workers, non-contact organic solvent industry workers, and trichlorethylene were collected. Triclosan was determined in urine samples by headspace-gas chromatography and in blood samples by immunoglobulin assay kit Serum levels of superoxide dismutase (SOD), malondialdehyde (MDA) in serum, glutathione peroxidase Enzyme (GSH-PX) activity. Results IgA, IgE and IgG levels did not change significantly, but the observation group and the proportion of IgM lower than the control group, a significant difference. Trichloroacetic acid in the observation group and case group of urine TCA higher than the control group, a significant difference. CD3 +, CD4 +, CD4 + / CD8 + in case group and observation group were lower than those in control group, and CD8 + was higher than that in control group. There were significant differences among the three groups. Compared with the control group, the levels of MDA, SOD and GSH-PX in observation group and case group were significantly increased, the difference was statistically significant. Conclusion Exposure to trichlorethylene alters the body’s immune system and increases lipid peroxidation in the body.