论文部分内容阅读
目的对BODIPY型一氧化氮探针检测巨噬细胞内一氧化氮的效果进行初步验证。方法采用免疫荧光染色和共聚焦显微镜对小鼠腹腔渗出液中的巨噬细胞的含量进行测定;采用BODIPY型探针对巨噬细胞内一氧化氮进行荧光染色,并激光扫描共聚焦显微镜对细胞内的荧光强度进行测定。结果液状石蜡诱生小鼠腹腔渗出细胞的CD14与CD68阳性细胞率分别为(32.33±7.27)%与(93.83±4.25)%,巨噬细胞为优势细胞;LPS刺激组与对照组细胞的荧光强度与面积比值分别为57.23±18.65和24.57±10.25,LPS刺激巨噬细胞的荧光强度与面积比值明显高于对照组细胞(P<0.01)。结论 BODIPY型一氧化氮探针可用于细胞一氧化氮荧光成像和巨噬细胞内一氧化氮检测。
Objective To verify the effect of BODIPY-type nitric oxide probe on the detection of nitric oxide in macrophages. Methods Immunofluorescence staining and confocal microscopy were used to determine the amount of macrophages in peritoneal exudate of mice. Fluorescent staining of nitric oxide in macrophages was performed by using BODIPY probe, and laser scanning confocal microscopy The intracellular fluorescence intensity was measured. Results The positive rates of CD14 and CD68 in peritoneal exudate cells induced by liquid paraffin were (32.33 ± 7.27)% and (93.83 ± 4.25)%, respectively. Macrophages were predominant cells. Fluorescence of LPS stimulated and control cells The ratio of intensity to area was 57.23 ± 18.65 and 24.57 ± 10.25 respectively. The ratio of fluorescence intensity to area of LPS-stimulated macrophages was significantly higher than that of control cells (P <0.01). Conclusion The BODIPY nitric oxide probe can be used to detect nitric oxide in macrophages.