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目的观察大鼠弥漫性轴索损伤(DAI)模型伤后不同时间大脑白质磁共振弥散张量成像(DTI)弥散参数和β-APP、NF68免疫组织化学染色的变化。方法建立SD大鼠Marmarou DAI模型,分别于伤后3、12、24、72 h行DTI扫描,获取大鼠胼胝体、内囊和外囊弥散参数;比较相应部位β-APP单位面积阳性轴索数及阳性染色面积百分比,NF68平均光密度的伤后变化。结果实验组胼胝体、内囊和外囊轴向弥散(AD)、各向异性分数(FA)伤后逐步下降;β-APP单位面积阳性轴索数量、阳性染色面积百分比及NF68平均光密度伤后逐步增加。相关分析证实AD、FA值与β-APP、NF68变化呈显著负相关。结论 DTI弥散参数可作为DAI早期诊断的生物标记物。
Objective To observe the changes of diffusion coefficient (DTI) and β-APP, NF68 immunohistochemical staining of cerebral white matter in different time after injury in rats with diffuse axonal injury (DAI). Methods The Marmarou DAI model of SD rats was established. DTI scan was performed at 3, 12, 24, and 72 hours after injury to obtain the corpus callosum, internal capsule and external capsule diffusion parameters of rats. The number of β-APP positive axons And the positive staining area percentage, NF68 average optical density changes after injury. Results In experimental group, the axial diffusion (AD) and anisodial score (FA) of corpus callosum, inner capsule and outer capsule decreased gradually; the number of positive axonal β-APP per unit area, the percentage of positive staining area and the average optical density of NF68 Gradually increase. Correlation analysis confirmed that AD, FA values and β-APP, NF68 changes were significantly negatively correlated. Conclusion DTI diffusion parameters can be used as early biomarkers for DAI diagnosis.