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The purpose of this study was to determine perfluoroalkyl substances (PFASs) in human serum,urine,hair and nail from general populations,and to investigate the possibility for human urine,hair and nail used as the biomonitoring sample for PFASs exposure.We detected the concentrations of PFHxA,PFOA,PFNA,PFDA,PFUnDA,PFDoA,PFHxS and PFOS in 39 matched human serum,urine,hair and nail samples from Shenzhen in China.The detection frequency and the median level of PFOS were all higher than that of the other PFASs in four matrices.The median concentration of PFOS in serum,urine,hair,and nail were 9.24 ng/mL,13.96 ng/L,0.58 ng/g and 0.63 ng/g,respectively.The results of spearman correlation test indicated that nail was an ideal matrix for biomonitoring PFOS rather than human urine and hair in general populations for the non-invasive sampling.