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取美州黄莲的不同时期的外植体进行组织培养。实验结果指出,只有幼胚、绿色胚芽叶(先出叶)和子叶才诱导出愈伤组织,并在绿色胚芽叶中直接诱导出植株。幼胚的培养在授粉后3～21天内,只有7天后的幼胚在有或无激素条件下才发育成完整植株。成苗率与胚的成熟度成正比。早期阶段的幼胚培养成苗与外源激素有一定关系。
Take the different states of Huanglian explants for tissue culture. The experimental results indicate that only the immature embryos, the green germ leaves (first leaves) and the cotyledons induced the callus, and the plants were directly induced in the green germ leaves. Immature embryos were cultured within 3 to 21 days after pollination. Only 7 days later, the immature embryos developed into complete plants with or without hormone. Seedling rate is proportional to embryo maturity. Early stage of embryo culture seedlings and exogenous hormones have a certain relationship.