论文部分内容阅读
目的探讨胃部疾病患者组织和血清中白细胞介素23(IL-23)、胃蛋白酶原1(PG1)的变化及临床意义。方法分别收集慢性浅表性胃炎、慢性萎缩性胃炎伴肠化生以及胃癌患者病变组织及外周血,免疫组织化学染色检测IL-23在这3种胃部疾病组织中的表达,时间分辨免疫荧光分析法定量检测血清PG1的水平,ELISA检测血清IL-23的水平,Pearson相关分析验证慢性萎缩性胃炎伴肠化生及胃癌患者血清中IL-23与PG1的相关性。结果在组织样本中,与慢性浅表性胃炎组相比,慢性萎缩性胃炎伴肠化生组和胃癌组IL-23表达明显增高。与慢性浅表性胃炎组相比,慢性萎缩性胃炎伴肠化生组和胃癌组血清IL-23浓度也明显升高,且呈递增趋势;而PG1浓度则在慢性萎缩性胃炎伴肠化生组和胃癌组中明显降低。Pearson相关性分析显示慢性萎缩性胃炎伴肠化生组和胃癌组中血清IL-23的水平与PG1呈负相关。结论慢性萎缩性胃炎伴肠化生和胃癌患者组织中IL-23高表达,且血清IL-23的水平与PG1呈负相关。
Objective To investigate the changes and clinical significance of interleukin-23 (IL-23) and pepsinogen-1 (PG1) in patients with gastric diseases. Methods Chronic superficial gastritis, chronic atrophic gastritis with intestinal metaplasia and gastric cancer tissues and peripheral blood were collected. The expression of IL-23 in the three gastric diseases tissues was detected by immunohistochemical staining. Time-resolved immunofluorescence Serum levels of PG1 were assayed by the method of analysis, the level of serum IL-23 was detected by ELISA, and the correlation between serum IL-23 and PG1 in patients with chronic atrophic gastritis and intestinal metaplasia and gastric cancer was verified by Pearson correlation analysis. Results Compared with chronic superficial gastritis group, the expression of IL-23 in chronic atrophic gastritis with intestinal metaplasia group and gastric cancer group was significantly increased in the tissue samples. Compared with chronic superficial gastritis group, serum IL-23 level in chronic atrophic gastritis with intestinal metaplasia group and gastric cancer group also increased significantly, and the increasing trend; PG1 concentration in chronic atrophic gastritis with intestinal metaplasia Group and gastric cancer significantly reduced. Pearson correlation analysis showed that serum IL-23 level in chronic atrophic gastritis with intestinal metaplasia group and gastric cancer group was negatively correlated with PG1. Conclusion The expression of IL-23 in patients with chronic atrophic gastritis complicated with intestinal metaplasia and gastric cancer is high, and the level of serum IL-23 is negatively correlated with PG1.