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[Objective] The purpose of this study was to investigate the effects and possible mechanisms of EtOAc extract from Gastrodia elata on oxidative damage of PC12cells induced by H2O2. [Methods]PC12cells were cultured and exposed to H2O2to establish oxidative damage model. The protective effect of EtOAc extract was observed by morphological identification,colorimetric MTT assay,leakage rate of LDH,intracellular ROS level and T-SOD activity. [Results]Compared with the model group,EtOAc extract from Gastrodia elata could improve the cells morphology significantly,increase the survival rate of 14. 7%(P < 0. 01),decrease the ROS level(P < 0. 01) and the release of LDH(P <0.01) from cells into culture medium,raise intracellular T-SOD activity(P <0.01). [Conclusions]EtOAc extract from Gastrodia elata had obviously antioxidant effect in vitro.
[Objective] The purpose of this study was to investigate the effects and possible mechanisms of EtOAc extract from Gastrodia elata on oxidative damage of PC12 cells induced by H2O2. [Methods] PC12 cells were cultured and exposed to H2O2 to establish oxidative damage model. The protective effect of EtOAc extract was observed by morphological identification, colorimetric MTT assay, leakage rate of LDH, intracellular ROS level and T-SOD activity. [Results] Compared with the model group, EtOAc extract from Gastrodia elata could improve the cells morphology significantly, increase the survival (P <0.01) and the release of LDH (P <0.01) from cells into culture medium, raising intracellular T-SOD activity (P <0.01) 0.01). [Conclusions] EtOAc extract from Gastrodia elata had obviously antioxidant effect in vitro.