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目的 :研究卵巢癌细胞中RNF123对p27Kip1蛋白稳定性的调控作用。方法 :流式细胞分析仪测定血清饥饿释放过程中SKOV3细胞的周期分布情况,利用Western blot检测该过程中干扰RNF123前后p27Kip1蛋白的表达水平。在SKOV3细胞中转染sictrl和si RNF123,Western blot检测p27Kip1的半衰期。结果:SKOV3细胞血清饥饿48 h,SKOV3细胞周期阻滞在G1/G0期,而血清释放后S期显著增加。在此过程中,p27Kip1表达下调。转染si RNF123组相较于sictrl组,p27Kip1蛋白水平增高。降低RNF123表达后,p27Kip1的半衰期延迟。结论 :在卵巢癌细胞中降低RNF123的表达能抑制p27Kip1的降解。
Objective: To study the regulatory effect of RNF123 on the stability of p27Kip1 protein in ovarian cancer cells. Methods: The cell cycle distribution of SKOV3 cells during serum starvation release was measured by flow cytometry. The expression of p27Kip1 protein was detected by Western blot before and after interference with RNF123. Sictrl and si RNF123 were transfected in SKOV3 cells and the half-life of p27Kip1 was detected by Western blot. RESULTS: After SKOV3 cells were starved for 48 hours, the cell cycle of SKOV3 cells was blocked in G1 / G0 phase, while S phase in serum was significantly increased. During this process, p27Kip1 expression is down-regulated. Transfection of si RNF123 group compared with the sictrl group, p27Kip1 protein levels increased. After decreasing RNF123 expression, the half-life of p27Kip1 is delayed. Conclusion: Decreasing the expression of RNF123 in ovarian cancer cells can inhibit the degradation of p27Kip1.