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目的筛选朝鲜白头翁Pulsatilla cernua的抗炎有效部位并研究其化学成分。方法将朝鲜白头翁干燥根以70%乙醇回流提取,提取物经大孔吸附树脂柱色谱洗脱,依次得到水及30%、50%、95%乙醇洗脱组分,通过二甲苯致小鼠耳廓肿胀实验以确定其抗炎有效部位;采用各种柱色谱分离纯化抗炎有效部位中的化合物,根据理化特征并结合波谱学数据分析鉴定化合物的结构。结果朝鲜白头翁根经大孔吸附树脂柱色谱,50%乙醇洗脱组分具有较强的抗炎活性,对其进行系统的化学成分研究,共分离得到12个化合物,分别鉴定为(+)-8-羟基松脂素-8-O-β-D-吡喃葡萄糖苷(1)、3,4:3′,4′-bis(methylenedioxy)-9′-hydroxyl-lignane-9-methyl-O-β-D-glucopyranoside(2)、prinsepiol-4-O-β-D-glucopyranoside(3)、(+)-环合橄榄树脂素-6-O-β-D-葡萄吡喃糖苷(4)、6-O-(E)-feruloyl-β-glucopyranoside(5)、6-O-(E)-feruloyl-α-glucopyranoside(6)、齐墩果酸-3-O-β-D-吡喃葡萄糖-(1→2)-β-D-吡喃葡萄糖-(1→3)-β-D-吡喃葡萄糖(7)、常春藤苷基-3-O-β-D-吡喃葡萄糖-(1→2)-β-D-吡喃葡萄糖-(1→3)-β-D-吡喃葡萄糖-(1→3)-β-D-吡喃葡萄糖(8)和常春藤苷基-3-O-β-D-吡喃葡萄糖-(1→2)-β-D-吡喃葡萄糖-(1→3)-β-D-吡喃葡萄糖(9)、齐墩果酸-3-O-β-D-吡喃葡萄糖-(1→2)-α-L-吡喃阿拉伯糖苷(10)、刺囊酸-3-O-β-D-吡喃葡萄糖-(1→2)-α-L-吡喃阿拉伯糖苷(11)和羽扇豆醇(12)。结论朝鲜白头翁50%乙醇洗脱组分为其抗炎有效部位,该部位中得到的化合物2~4、6、8和9为首次从白头翁属植物中分离得到。
Objective To screen the anti-inflammatory active fractions of Pulsatilla cernua from North Korea and study its chemical constituents. Methods The dried roots of Pulsatilla sinicus were extracted with 70% ethanol and the extracts were eluted by macroporous resin column chromatography. The fractions were eluted with water and 30%, 50%, 95% Swelling test to determine its anti-inflammatory active site; using a variety of column chromatography purification compounds anti-inflammatory active site, based on physical and chemical characteristics and combined with spectroscopic data analysis identified the structure of the compound. Results Pulsatilla striata had strong anti-inflammatory activity by macroporous resin column chromatography and eluted with 50% ethanol. The chemical constituents were studied systematically. Twelve compounds were isolated and identified as (+) - Hydroxy-lignane-9-methyl-O-methyl-O-β-D-glucopyranoside (1) β-D-glucopyranoside (2), prinsepiol-4-O-β-D-glucopyranoside (3) 6-O- (E) -feruloyl-β-glucopyranoside (5), 6-O- (E) -feruloyl- α-glucopyranoside (6), oleanolic acid -3-O-β-D-glucopyranose - (1 → 2) -β-D-glucopyranosyl- (1 → 3) -β-D-glucopyranose (7) 1 → 2) -β-D-glucopyranosyl- (1 → 3) -β-D-glucopyranose- (1 → 3) -β-D-glucopyranose (8) (1 → 2) -β-D-glucopyranose- (1 → 3) -β-D-glucopyranose (9), oleanolic acid-3-O β-D-glucopyranosyl- (1 → 2) -α-L-arabinopyranoside (10), Echinocystic acid -3-O-β-D-glucopyranosyl- (1 → 2) -α -L-pyran Arab Glycoside (11) and lupeol (12). Conclusions Pulsatilla chinensis is eluted by 50% ethanol and its anti-inflammatory active site is obtained. Compounds 2 ~ 4, 6, 8 and 9 obtained in this site were isolated from Pulsatilla for the first time.