酵母菌是工業上重要的微生物之一。我國在這方面的工作正在開展。在酵母菌的工作中如優良品系的選擇或品種間的雜交,常應用單細胞及單孢子分離的方法來培養。酵母菌的分離方法,正如其他微生物一樣,大體上可分為兩種:“即稀釋平皿’和”懸滴顯微操縱法“。後者應用機械操作,在效果上有高度的準確性,尤其在雜交工作上用顯微操縱法更為必要。所以過去許多學者都廣泛採用這種方法。例如Lindegren就用deFonbrune顯微操縱器作酵母的雜交工作;Косиков就採用Péterfi顯微操縱器来作 Yeast is one of the industrially important microorganisms. My country’s work in this area is under way. In the work of yeast, such as the choice of good lines or cross between varieties, often single cell and single spore separation methods to culture. Yeast isolation methods, just like any other microorganism, can be broadly classified into two types: ”ie,“ a dilution plate ”and“ hanging drop micromanipulation method. ”The latter uses mechanical manipulation with a high degree of accuracy in performance , Especially in the work of hybrid microscopy is more necessary, so many scholars in the past are widely used this method.For example, Lindegren on deFonbrune micromanipulator for the hybridization of yeast; Косиков Péterfi micromanipulator Make